Biolipidure®

Synthetic BSA Substitutes

Biolipidure®

Biolipidure® enhances sensitivity of immunoassays.

Biolipidure® has variations with different molecular weights, hydrophilic–hydrophobic ratios, and ionic properties. Several grades should be tested empirically to determine which performs best in a given assay.

Biolipidure® — Additives for Diagnostics

For Enhancement of Sensitivity

Features

• Biolipidure® enhances sensitivity and accuracy.
• Biolipidure® suppresses non-specific adsorption.
• Biolipidure® stabilizes antibodies and enzymes.
• Biolipidure® eliminates lot-to-lot variations.
• Biolipidure® does not require biohazardous handling.
• Biolipidure® is provided as a 5 wt% aqueous solution with no preservatives.

How to Use

• Prepare a buffer solution with 1 wt% Biolipidure®-405.
• Dissolve the sample (e.g., mucosa) in the prepared buffer.
• Load the diluted sample onto the sample pad of the immunochromatograph.

Enhancement of Sensitivity

Name	Property	HLB	Surface tension*	Volume	Price
Biolipidure®-103	Amphoteric	13.4	72.4
Biolipidure®-405	Anionic	12.1	73.1
Biolipidure®-802	Amphoteric	10.9	44.7

*Surface tension (dyn/cm): at a conc. of 2 wt% in water

Biolipidure® — Additives for Diagnostics

For Suppression of Non-Specific Adsorption

Features

• Enhances sensitivity and accuracy.
• Suppresses non-specific adsorption.
• Stabilizes antibodies and enzymes.
• Eliminates lot-to-lot variations.
• Does not require biohazardous handling.
• Provided as 5 wt% aqueous solution without preservatives.

How to Use

• Add Biolipidure® to 4 wt% in the final working solution.

Name	Property	HLB	Surface tension*	Volume	Price
Biolipidure®-203	Amphoteric	12.6	52.8
Biolipidure®-206	Anionic	9.6	39.1
Biolipidure®-802	Amphoteric	10.9	44.7

*Surface tension (dyn/cm): at a conc. of 2 wt% in water

Biolipidure® — Additives for Diagnostics

For Stabilization of Antibodies and Enzymes

Features

• Enhances sensitivity and accuracy.
• Suppresses non-specific adsorption.
• Stabilizes antibodies and enzymes.
• Eliminates lot-to-lot variations.
• Does not require biohazardous handling.
• Provided as 5 wt% aqueous solution with no preservatives.

How to Use

• Add Biolipidure® to 4 wt% in the final working solution.

Stabilization of Immobilized Antibody

Name	Property	HLB	Surface tension*	Volume	Price
Biolipidure®-203	Amphoteric	12.6	52.8
Biolipidure®-206	Anionic	9.6	39.1
Biolipidure®-802	Amphoteric	10.9	44.7

*Surface tension (dyn/cm): at a conc. of 2 wt% in water

Lipidure®-GD

LIPIDURE®-GD series

new additives suitable for PCR application

Feature of LIPIDURE®-GD

Efficacy
+ : high
++ : higher

Value

• Enable challenging detection of tiny amounts of the target by PCR systems
• Save time on PCR system optimization

Function

By simply adding LIPIDURE®-GD into your master mix, the PCR system achieves:

• Stronger end-point signal
• Improved limit of detection
• Better preservation stability

Sensitizing in PCR

[Enhancing the end-point signal]

Evaluation

1. A commercially available COVID-19 RT-qPCR kit (Japanese NIID method) was prepared.
2. LIPIDURE®-GD100 or LIPIDURE®-GD200 was added to the master mix (2 vol% final for GD100, 1 vol% final for GD200).
3. Control RNA (50 copies of set No. 2) was added.
4. One-step RT-qPCR was conducted.

[Improving the limit of detection]

Evaluation

1. A commercially available COVID-19 RT- qPCR kit (Japanese NIID method) was conducted with LIPIDURE®-GD100 added to the master mix (2 vol% final as GD100).
2. Positive control RNA (N set No.2) and Negative control (PR grade purified water) were used.
3. The result was deemed to be positive (+) when the amplification plot exceeded the threshold, otherwise to be negative (-).

LIPIDURE®-GD significantly improves the limit of detection.

[Comparison among LIPIDURE®-GD]

Evaluation

1. A commercially available COVID-19 RT-qPCR kit (Japanese NIID method) was conducted with LIPIDURE*-GD added to the master mix.
2. Positive control RNA (500 copies/test of N set No.2)

The offoct on amplification curve by adding each LIPIDURE®-GD was ovaluated. Bost product may vary with individual PCR systom.

[DNA Amplification Enhancement]

An agarose gel electrophoresis was conducted to confirm whether LIPIDURE®-GD enhances DNA amplification itself, or only enhances the fluorescent signal.

Evaluation

• A commercially available COVID-19 RT-qPCR kit (Japanese NIID method) was prepared.
• LIPIDURE®-GD100 was added to the master mix (2 vol% final concentration as GD100).
• Positive control RNA (200 copies/test of N set No.2) was added.
• One-step RT-qPCR was conducted.
• The crude RT-qPCR product was analyzed by agarose gel electrophoresis.

Apply volume: 5 µL
Gel / Buffer: 3% (w/v) agarose / 0.5× TBE

Amplification of the target fragment can be significantly increased by using LIPIDURE®-GD.

Hypothetical Mechanism of LIPIDURE®-GD

(A) Interaction with DNA Polymerase

• LIPIDURE® can block the adsorption of protein onto the surface of the reaction tube.
• LIPIDURE® may enhance the enzyme activity of DNA polymerase through a molecular crowding effect.

(B) Interaction with Nucleic Acid

• LIPIDURE® polymer contains a chaotropic phosphorylcholine (PC) group.
• The chaotropic group may accelerate denaturation of template RNA and improve the specificity of primers and probes by weakening hydrogen bonds between Watson–Crick base pairs.

When a primer anneals to the target region of the template, a perfect match of the nucleic acid sequence is required to form sufficiently strong affinity to maintain hybridization. As a result, even if the PC group weakens hydrogen bonding between Watson–Crick base pairs, specific hybridization can still be maintained, leading to improved specificity.

Case Study

This section presents a case study on sensitivity enhancement of a PCR system.

STEP 1: Selection of LIPIDURE®-GD

Evaluation

• A commercially available COVID-19 RT-qPCR kit (a modified Japanese NIID method) was prepared.
• LIPIDURE®-GD was added to the master mix (1.0 vol% final concentration).
• Positive control RNA (500 copies/test of N set No.2) or a negative control was added.
• One-step RT-qPCR was conducted to compare the amplification plots.

The most suitable LIPIDURE®-GD for this PCR system was GD400 in terms of end-point enhancement.

The optimal LIPIDURE®-GD polymer may vary depending on the individual PCR system.

STEP 2: Confirmation of the Effect of LIPIDURE®-GD400

In this case, LIPIDURE®-GD400 enhanced the sensitivity of the PCR system, and a better limit of detection was observed.

STEP 3: Additional Effect of LIPIDURE®-GD400

In this case, LIPIDURE®-GD400 also enhanced the quantifiability and the amplifying efficiency of the PCR system.